Stress-induced alternative splicing of Serine/Arginine-rich proteins in the moss Physcomitrella patens

Serine/Arginine-rich proteins in Physcomitrella patens

Genome-Wide Analysis of Heat-Sensitive Alternative Splicing in Physcomitrella patens.

Plant growth and development are constantly influenced by temperature fluctuations. To respond to temperature changes, different levels of gene regulation are modulated in the cell. Alternative splicing (AS) is a widespread mechanism increasing transcriptome complexity and proteome diversity. Although genome-wide studies have revealed complex AS patterns in plants, whether AS impacts the stress...

Isolation and regeneration of protoplasts of the moss Physcomitrella patens.

This method is adapted from a protocol described by Grimsley et al. (1977). For more information about P. patens as a model organism, see The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies (Cove et al. 2009a). For details about the growth of P. patens on cellophane overlay plates, see Culturing the Moss Physcomitrella patens (Cove et al. 2009b). For p...

The Mkk3/6-p38–Signaling Cascade Alters the Subcellular Distribution of Hnrnp A1 and Modulates Alternative Splicing Regulation

Individual members of the serine-arginine (SR) and heterogeneous nuclear ribonucleoprotein (hnRNP) A/B families of proteins have antagonistic effects in regulating alternative splicing. Although hnRNP A1 accumulates predominantly in the nucleus, it shuttles continuously between the nucleus and the cytoplasm. Some but not all SR proteins also undergo nucleo-cytoplasmic shuttling, which is affect...

Proteomic studies of the abiotic stresses response in model moss – Physcomitrella patens

Moss species Physcomitrella patens has been used as a model system in plant science for several years, because it has a short life cycle and is easy to be handled. With the completion of its genome sequencing, more and more proteomic analyses were conducted to study the mechanisms of P. patens abiotic stress resistance. It can be concluded from these studies that abiotic stresses could lead to ...